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TAGLN Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TAGLN Antibody (C-term) AP6678b
    Western blot analysis of TAGLN antibody (C-term) (Cat. #AP6678b) in mouse bladder tissue lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab.
    detail
  • IHC-P - TAGLN Antibody (C-term) AP6678b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TAGLN Antibody (C-term) AP6678b
    TAGLN Antibody (C-term) (Cat. #AP6678b) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q01995
Other Accession Q9TS87
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22611 Da
Antigen Region 139-167 aa
Additional Information
Gene ID 6876
Other Names Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha, TAGLN, SM22, WS3-10
Target/Specificity This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-167 amino acids from the C-terminal region of human TAGLN.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAGLN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAGLN
Synonyms SM22, WS3-10
Function Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

References

Zhao,L., Mod. Pathol. 22 (6), 786-796 (2009) Leguillette,R., Am. J. Respir. Crit. Care Med. 179 (3), 194-204 (2009) Yu,H., FASEB J. 22 (6), 1778-1789 (2008)

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$ 365.00
$ 140.00
Cat# AP6678b
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