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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   TBP Antibody (C-term)   

TBP Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TBP Antibody (C-term) AP6680b
    Western blot analysis of TBP antibody (C-term) (Cat. #AP6680b) in HL60 cell line lysates (35ug/lane). TBP (arrow) was detected using the purified Pab.
  • IHC-P - TBP Antibody (C-term) AP6680b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with TBP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - TBP Antibody (C-term) AP6680b
    Confocal immunofluorescent analysis of TBP Antibody (C-term)(Cat#AP6680b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20226
Other Accession P27633, P29037, Q4R4F5, O13270, Q2HJ52
Reactivity Human
Predicted Bovine, Chicken, Monkey, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37698 Da
Antigen Region 301-330 aa
Additional Information
Gene ID 6908
Other Names TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP, GTF2D1, TF2D, TFIID
Target/Specificity This TBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the C-terminal region of human TBP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBP
Synonyms GTF2D1, TF2D, TFIID
Function General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Cellular Location Nucleus.
Tissue Location Widely expressed, with levels highest in the testis and ovary.
Research Areas
Citations (0)

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Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.


Ohi,K., Genes Brain Behav. 8 (4), 473-480 (2009)
Reid,S.J., Brain Res. 1268, 190-199 (2009)

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Cat# AP6680b
(40 western blots)
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