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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   WNT4 Antibody (Center)   

WNT4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • FC - WNT4 Antibody (Center) AP6683C
    Overlay histogram showing MCF-7 cells stained with AP6683c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6683c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - WNT4 Antibody (Center) AP6683C
    All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: mouse bladder lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - WNT4 Antibody (Center) AP6683C
    All lanes : Anti-WNT4 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: human testis lysate Lane 3: mouse bladder lysate Lane 4: rat lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - WNT4 Antibody (Center) AP6683C
    Formalin-fixed and paraffin-embedded human bladder carcinoma reacted with WNT4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56705
Other Accession Q9QXQ5, P22724
Reactivity Human, Mouse, Rat
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39052 Da
Antigen Region 211-239 aa
Additional Information
Gene ID 54361
Other Names Protein Wnt-4, WNT4
Target/Specificity This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-239 amino acids from the Central region of human WNT4.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT4
Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations ( 0 )


The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.


Kuulasmaa,T., Horm. Metab. Res. 40 (10), 668-673 (2008)
Miyakoshi,T., Endocr. Pathol. 19 (4), 261-273 (2008)

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$ 295.00
$ 99.00
Cat# AP6683C
(40 western blots)
Availability: In Stock
Bulk Size
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