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CCL2 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6699b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP6699b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CCL2 Antibody (C-term) - Product info | |
| Application | WB, IHC, IF, FC
|
| Primary Accession | P13500 |
| Other Accession | P61274 |
| Reactivity | Human |
| Predicted | Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 11025 Da |
CCL2 Antibody (C-term) - Additional info | |
| Gene ID 6347 | |
| Other Names CCL2; MCP1; SCYA2; C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytokine A2 | |
| Target/Specificity This CCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68~98 amino acids from the C-terminal region of human CCL2. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CCL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CCL2 Antibody (C-term) - Protein Information | |
| Name CCL2 | |
| Synonyms MCP1, SCYA2 | |
| Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis | |
| Cellular Location Secreted. | |
CCL2 Antibody (C-term) - Related products
AP6699b: CCL2 Antibody (C-term)
DC02741: Human CCL2 cDNA Clone
CCL2 Antibody (C-term) - Application data
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Western blot analysis of CCL2 Antibody (C-term) (Cat. #AP6699b) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue with CCL2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Immunofluorescence analysis of CCL2 Antibody (C-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
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Confocal immunofluorescent analysis of CCL2 Antibody (C-term) (Cat. #AP6699b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of hela cells using CCL2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCL2 Antibody (C-term) - Research Areas
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BACKGROUND
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
REFERENCES
Saenz Lopez,P., Actas Urol Esp 33 (5), 474-481 (2009) Rollins,B.J., Genomics 10 (2), 489-492 (1991)
