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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KRT14 Antibody (Center)   

KRT14 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KRT14 Antibody (Center) AP6713c
    Anti-KRT14 Antibody (Center) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - KRT14 Antibody (Center) AP6713c
    Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - KRT14 Antibody (Center) AP6713c
    KRT14 Antibody (Center) (Cat.#AP6713c) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02533
Other Accession Q6IFU7, Q6IFX2, Q6IFU8, Q9QWL7, Q04695, A1L595, Q6IFV1, Q61781
Reactivity Human, Mouse
Predicted Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51561 Da
Antigen Region 276-303 aa
Additional Information
Gene ID 3861
Other Names Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Target/Specificity This KRT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-303 amino acids from the Central region of human KRT14.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT14
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Cellular Location Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern
Tissue Location Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Research Areas
Citations (0)

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KRT14 is a member of the keratin family, the most diverse group of intermediate filaments. This protein, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.


Barcelos,A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009) Romano,R.A., PLoS ONE 4 (5), E5623 (2009)

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$ 295.00
$ 99.00
Cat# AP6713c
(40 western blots)
Availability: In Stock
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