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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   F10 Antibody (C-term)   

F10 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - F10 Antibody (C-term) AP6728b
    Western blot analysis of F10 antibody (C-term) (Cat. #AP6728b) in 293 cell line lysates (35ug/lane). F10 (arrow) was detected using the purified Pab.
  • IHC-P - F10 Antibody (C-term) AP6728b
    F10 Antibody (C-term) (Cat. #AP6728b) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the F10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - F10 Antibody (C-term) AP6728b
    F10 Antibody (C-term) (Cat. #AP6728b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00742
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54732 Da
Antigen Region 351-381 aa
Additional Information
Gene ID 2159
Other Names Coagulation factor X, Stuart factor, Stuart-Prower factor, Factor X light chain, Factor X heavy chain, Activated factor Xa heavy chain, F10
Target/Specificity This F10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-381 amino acids from the C-terminal region of human F10.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsF10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name F10
Function Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Cellular Location Secreted.
Tissue Location Plasma; synthesized in the liver.
Research Areas
Citations (0)

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F10 is the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting.


Alba,R., Blood 114 (5), 965-971 (2009)
Borensztajn,K., Thromb. Res. 124 (2), 219-225 (2009)
Larson,P.J., Biochemistry 37 (14), 5029-5038 (1998)

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Cat# AP6728b
(40 western blots)
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