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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   HPRT1 Antibody (N-term)   

HPRT1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HPRT1 Antibody (N-term) AP6732A
    Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (N-term) (Cat. #AP6732a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene.
  • IHC-P - HPRT1 Antibody (N-term) AP6732A
    HPRT1 Antibody (N-term) (Cat. #AP6732a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPRT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HPRT1 Antibody (N-term) AP6732A
    HPRT1 Antibody (N-term) (Cat. #AP6732a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00492
Other Accession P27605, Q45FY6, P00493, Q6LDD9, P00494
Reactivity Human
Predicted Hamster, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24579 Da
Antigen Region 65-94 aa
Additional Information
Gene ID 3251
Other Names Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT
Target/Specificity This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human HPRT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHPRT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HPRT1
Synonyms HPRT
Function Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.


Ceballos-Picot,I., Hum. Mol. Genet. 18 (13), 2317-2327 (2009)
Sculley,D.G., Hum. Genet. 90 (3), 195-207 (1992)

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Cat# AP6732A
(40 western blots)
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