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Fascin Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Fascin Antibody (Center) AP6736c
    Western blot analysis of Fascin Antibody (Center) (Cat. #AP6736c) in Y79 cell line lysates (35ug/lane). Fascin (arrow) was detected using the purified Pab.
    detail
  • IHC-P - Fascin Antibody (Center) AP6736c
    Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - Fascin Antibody (Center) AP6736c
    Flow cytometric analysis of NCI-H292 cells using Fascin Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q16658
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54530 Da
Antigen Region 322-350 aa
Additional Information
Gene ID 6624
Other Names Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, FSCN1, FAN1, HSN, SNL
Target/Specificity This Fascin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the Central region of human Fascin.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFascin Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FSCN1
Synonyms FAN1, HSN, SNL
Function Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945). Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786).
Cellular Location Cytoplasm, cytosol. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell projection, filopodium. Cell projection, invadopodium. Cell projection, microvillus. Cell junction. Note=Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F- actin at the transitional domain of the axonal growth cone (By similarity). {ECO:0000250|UniProtKB:Q61553, ECO:0000269|PubMed:21706053}
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.

References

Qualtrough,D., Br. J. Cancer 101 (7), 1124-1129 (2009)
Ono,S., J. Biol. Chem. 272 (4), 2527-2533 (1997)

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$ 365.00
$ 140.00
Cat# AP6736c
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