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GBAS Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GBAS Antibody (Center) AP6752c
    Western blot analysis of GBAS Antibody (Center) (Cat. #AP6752c) in Ramos cell line lysates (35ug/lane).GBAS (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GBAS Antibody (Center) AP6752c
    GBAS Antibody (Center) (Cat. #AP6752c) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GBAS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - GBAS Antibody (Center) AP6752c
    GBAS Antibody (Center) (Cat. #AP6752c) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O75323
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33743 Da
Antigen Region 130-159 aa
Additional Information
Gene ID 2631
Other Names Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence, GBAS, NIPSNAP2
Target/Specificity This GBAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the Central region of human GBAS.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGBAS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NIPSNAP2 (HGNC:4179)
Synonyms GBAS
Function May act as a positive regulator of L-type calcium channels.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O55126}. Mitochondrion outer membrane
Tissue Location Widely expressed. Most abundant in heart and skeletal muscle
Research Areas
Citations ( 0 )

Background

Chromosomal region 7p12, which contains GBAS, is amplified in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumor.The predicted 286-amino acid protein contains a signal peptide, a transmembrane domain, and 2 tyrosine phosphorylation sites. The GBAS transcript is expressed most abundantly in heart and skeletal muscle. GBAS protein might be involved in vesicular transport.

References

Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Seroussi, E., et al. Gene 212(1):13-20(1998)
Wang, X.Y., et al. Genomics 49(3):448-451(1998)

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$ 182.50
$ 70.00
Cat# AP6752c
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