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Fascin-3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Fascin-3 Antibody (N-term) AP6757c
    Anti-Fascin-3 Antibody (N-term) at 1:8000 dilution + human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - Fascin-3 Antibody (N-term) AP6757c
    Western blot analysis of Fascin-3 Antibody (N-term) (Cat. #AP6757c) in MDA-MB231, NCI-H460 cell line and mouse testis tissue lysates (35ug/lane). Fascin-3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - Fascin-3 Antibody (N-term) AP6757c
    Fascin-3 Antibody (N-term) (Cat. #AP6757c) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Fascin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - Fascin-3 Antibody (N-term) AP6757c
    Fascin-3 Antibody (N-term) (Cat. #AP6757c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9NQT6
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56624 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 29999
Other Names Fascin-3, Testis fascin, FSCN3
Target/Specificity This Fascin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Fascin-3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFascin-3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FSCN3
Function Acts as an actin bundling protein.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Expressed in testis.
Research Areas
Citations (0)
citation

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Background

FSCN3 acts as an actin bundling protein.

References

Tubb,B., et.al., Exp. Cell Res. 275 (1), 92-109 (2002)

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$ 365.00
$ 140.00
Cat# AP6757c
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