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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MAPRE1 Antibody (N-term)   

MAPRE1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MAPRE1 Antibody (N-term) AP6770a
    MAPRE1 Antibody (N-term) (Cat. #AP6770a) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the MAPRE1 antibody detected MAPRE1 protein (arrow).
  • IHC-P - MAPRE1 Antibody (N-term) AP6770a
    MAPRE1 antibody (N-term) (Cat. #AP6770a) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPRE1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - MAPRE1 Antibody (N-term) AP6770a
    MAPRE1 Antibody (N-term) (Cat. #AP6770a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15691
Other Accession Q7ZXP1, Q3B8Q0, Q8R001, Q15555, Q5ZKK1, Q3SZP2, Q66HR2, Q61166, Q5ZLC7, Q3ZBD9, NP_036457.1
Reactivity Human, Mouse
Predicted Bovine, Chicken, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29999 Da
Antigen Region 44-71 aa
Additional Information
Gene ID 22919
Other Names Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
Target/Specificity This MAPRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the N-terminal region of human MAPRE1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAPRE1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.


Jaulin, F., et al. J. Cell Biol. 190(3):443-460(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
De Groot, C.O., et al. J. Biol. Chem. 285(8):5802-5814(2010)
Jiang, K., et al. EMBO Rep. 10(8):857-865(2009)
Honnappa, S., et al. Cell 138(2):366-376(2009)

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Cat# AP6770a
(40 western blots)
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