- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
MAPRE1 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP6770b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MAPRE1 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | Q15691 |
| Other Accession | Q66HR2, Q61166, Q5ZLC7, Q3ZBD9 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 29999 Da |
MAPRE1 Antibody (C-term) - Additional info | |
| Gene ID 22919 | |
| Other Names MAPRE1; Microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; End-binding protein 1 | |
| Target/Specificity This MAPRE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196~225 amino acids from the C-terminal region of human MAPRE1. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MAPRE1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MAPRE1 Antibody (C-term) - Protein Information | |
| Name MAPRE1 | |
| Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration | |
| Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome | |
| Tissue Location Ubiquitously expressed. | |
MAPRE1 Antibody (C-term) - Related products
AP6770a: MAPRE1 Antibody (N-term)
AP6770b: MAPRE1 Antibody (C-term)
AP6770c: MAPRE1 Antibody (Center)
AP6770e: MAPRE1 Antibody (C-term)
RI13204: MAPRE1 predesign siRNA
DC09571: Human MAPRE1 cDNA Clone
LY12205a: MAPRE1 Over-expression Lysate
BP6770a: MAPRE1 Antibody (N-term) Blocking peptide
BP6770b: MAPRE1 Antibody (C-term) Blocking Peptide
MAPRE1 Antibody (C-term) - Application data
-
Western blot analysis of MAPRE1 Antibody (C-term) (Cat. #AP6770b) in HL-60, T47D cell line lysates (35ug/lane). MAPRE1 (arrow) was detected using the purified Pab.
-
Western blot analysis of MAPRE1 (arrow) using rabbit polyclonal MAPRE1 Antibody (C-term) (Cat. #AP6770b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPRE1 gene (Lane 2) .
-
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MAPRE1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
MAPRE1 Antibody (C-term) (Cat. #AP6770b) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MAPRE1 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability.
REFERENCES
Komarova,Y., J. Cell Biol. 184 (5), 691-706 (2009)
