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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GATA2 Antibody (C-term)   

GATA2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GATA2 Antibody (C-term) AP6772b
    Western blot analysis of GATA2 Antibody (C-term) (Cat. #AP6772b) in K562 cell line lysates (35ug/lane). GATA2 (arrow) was detected using the purified Pab.
  • FC - GATA2 Antibody (C-term) AP6772b
    Flow cytometric analysis of K562 cells using GATA2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - GATA2 Antibody (C-term) AP6772b
    Western blot analysis of GATA2 (arrow) using rabbit polyclonal GATA2 Antibody (C-term) (Cat. #AP6772b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA2 gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P23769
Other Accession P23824
Reactivity Human
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50500 Da
Antigen Region 380-407 aa
Additional Information
Gene ID 2624
Other Names Endothelial transcription factor GATA-2, GATA-binding protein 2, GATA2
Target/Specificity This GATA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human GATA2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGATA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATA2
Function Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Cellular Location Nucleus.
Tissue Location Endothelial cells.
Research Areas
Citations (0)

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Background

GATA2 is a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. This protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages.

References

Pan,X., et.al., J. Biochem. 127 (1), 105-112 (2000)

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Cat# AP6772b
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(40 western blots)
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