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Ku70 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6775a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Ku70 Antibody (N-term) - Product info | |
| Application | WB
|
| Primary Accession | P12956 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 69843 Da |
Ku70 Antibody (N-term) - Additional info | |
| Gene ID 2547 | |
| Other Names XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 6 | |
| Target/Specificity This Ku70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~46 amino acids from the N-terminal region of human KU70. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Ku70 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Ku70 Antibody (N-term) - Protein Information | |
| Name XRCC6 | |
| Synonyms G22P1 | |
| Function Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose- 5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription | |
| Cellular Location Nucleus. Chromosome. | |
Ku70 Antibody (N-term) - Related products
AP5089b: XRCC6 Antibody (C-term)
AP5089c: XRCC6 Antibody (Center)
AP6775a: Ku70 Antibody (N-term)
AP6775c: Ku70 Antibody (Center)
RI15763: XRCC6 predesign siRNA
DC02375: Human XRCC6 cDNA Clone
LY10971a: XRCC6 Over-expression Lysate
BP5089b: XRCC6 Antibody (C-term) Blocking Peptide
BP5089c: XRCC6 Antibody (Center) Blocking peptide
BP6775a: Ku70 Antibody (N-term) Blocking Peptide
BP6775c: Ku70 Antibody (Center) Blocking Peptide
AT2136a: G22P1 Antibody (monoclonal) (M01)
Ku70 Antibody (N-term) - Application data
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Western blot analysis of Ku70 Antibody (N-term) (Cat. #AP6775a) in A2058, NCI-H460 cell line lysates (35ug/lane). KU70 (arrow) was detected using the purified Pab.
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Western blot analysis of Ku70 (arrow) using rabbit polyclonal Ku70 Antibody (N-term) (Cat. #AP6775a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ku70 gene (Lane 2) (Origene Technologies).
Ku70 Antibody (N-term) - Research Areas
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BACKGROUND
Ku70 is a single stranded DNA-dependent ATP-dependent helicase. It has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
REFERENCES
Beskow,C., et.al., Br. J. Cancer 101 (5), 816-821 (2009) Tseng,R.C., et.al., Cancer 115 (13), 2939-2948 (2009)
