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ACLY Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6784b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ACLY Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P53396 |
| Other Accession | Q32PF2 |
| Reactivity | Human |
| Predicted | Bovine |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 120839 Da |
ACLY Antibody (C-term) - Additional info | |
| Gene ID 47 | |
| Other Names ACLY; ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; Citrate cleavage enzyme | |
| Target/Specificity This ACLY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1032~1062 amino acids from the C-terminal region of human ACLY. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACLY Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACLY Antibody (C-term) - Protein Information | |
| Name ACLY | |
| Function ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine | |
| Cellular Location Cytoplasm. | |
ACLY Antibody (C-term) - Related products
AP6784a: ACLY Antibody (N-term)
AP6784b: ACLY Antibody (C-term)
BP6784a: ACLY Antibody (N-term) Blocking Peptide
AJ1067a: ATP-citrate Antibody (C-term)
ACLY Antibody (C-term) - Application data
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Western blot analysis of ACLY Antibody (C-term) (Cat. #AP6784b) in Hela cell line lysates (35ug/lane). ACLY (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ACLY Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of hela cells using ACLY Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ACLY Antibody (C-term) - Research Areas
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BACKGROUND
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
REFERENCES
Wellen,K.E., et.al., Science 324 (5930), 1076-1080 (2009)
