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FUCA1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FUCA1 Antibody (Center) AP6791c
    Western blot analysis of FUCA1 Antibody (Center) (Cat. #AP6791c) in HepG2 cell line lysates (35ug/lane). FUCA1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - FUCA1 Antibody (Center) AP6791c
    FUCA1 Antibody (Center) (Cat. #AP6791c) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUCA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - FUCA1 Antibody (Center) AP6791c
    FUCA1 Antibody (Center) (Cat. #AP6791c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P04066
Other Accession Q60HF8
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53689 Da
Antigen Region 265-294 aa
Additional Information
Gene ID 2517
Other Names Tissue alpha-L-fucosidase, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase 1, FUCA1
Target/Specificity This FUCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human FUCA1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFUCA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FUCA1 (HGNC:4006)
Function Alpha-L-fucosidase is responsible for hydrolyzing the alpha- 1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Cellular Location Lysosome.
Research Areas
Citations (0)
citation

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Background

FUCA1 is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.

References

Palmieri,R.T., et.al., Cancer Epidemiol. Biomarkers Prev. 17 (12), 3567-3572 (2008)

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$ 182.50
$ 70.00
Cat# AP6791c
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