|Application ||WB, IHC-P, FC, E|
|Calculated MW||75208 Da|
|Antigen Region||494-523 aa|
|Other Names||Alkylated DNA repair protein alkB homolog 8, 11411-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8, ALKBH8, ABH8|
|Target/Specificity||This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALKBH8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly cytoplasmic|
|Tissue Location||Widely expressed, with highest expression in spleen, followed by pancreas and lung|
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Provided below are standard protocols that you may find useful for product applications.
ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis.
Shimada,K., et.al., Cancer Res. 69 (7), 3157-3164 (2009)
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