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TPTEa Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TPTEa Antibody (N-term) AP6811a
    TPTEa Antibody (E57) (Cat. #AP6811a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TPTEa antibody detected the TPTEa protein (arrow).
  • IHC-P - TPTEa Antibody (N-term) AP6811a
    TPTEa Antibody (N-term) (Cat. #AP6811a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TPTEa Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56180
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64322 Da
Antigen Region 42-74 aa
Additional Information
Gene ID 7179
Other Names Putative tyrosine-protein phosphatase TPTE, Cancer/testis antigen 44, CT44, Transmembrane phosphatase with tensin homology, Tumor antigen BJ-HCC-5, TPTE
Target/Specificity This TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human TPTEa.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPTEa Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Could be involved in signal transduction.
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Exclusively expressed in testis.
Research Areas
Citations (0)

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Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.


Hattori, M., et al., Nature 405(6784):311-319 (2000).
Chen, H., et al., Hum. Genet. 105(5):399-409 (1999).

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Cat# AP6811a
(40 western blots)
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