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SMAC Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6820b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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SMAC Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | Q9NR28 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 27131 Da |
SMAC Antibody (C-term) - Additional info | |
| Gene ID 56616 | |
| Other Names DIABLO; SMAC; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase | |
| Target/Specificity This SMAC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210~239 amino acids from the C-terminal region of human SMAC. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SMAC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SMAC Antibody (C-term) - Protein Information | |
| Name DIABLO | |
| Synonyms SMAC | |
| Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance | |
| Cellular Location Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis | |
| Tissue Location Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed | |
SMAC Antibody (C-term) - Related products
AP6820b: SMAC Antibody (C-term)
RI11506: DIABLO predesign siRNA
DC03233: Human DIABLO cDNA Clone
BP6820b: SMAC Antibody (C-term) Blocking Peptide
SMAC Antibody (C-term) - Application data
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Western blot analysis of SMAC Antibody (C-term) (Cat. #AP6820b) in T47D cell line lysates (35ug/lane). SMAC (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human testis tissue reacted with SMAC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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SMAC Antibody (C-term) (Cat. #AP6820b) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMAC Antibody (C-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SMAC is an inhibitor of apoptosis protein (IAP)-binding protein. This mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs.
REFERENCES
Carbone,A., et.al., Genes Chromosomes Cancer 47 (12), 1067-1075 (2008)
