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ACADM Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # Size Availability Price  
AP6827b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP6827b-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
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ACADM Antibody (C-term) - Product info

ApplicationWB, FC, IF, IHC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP11310
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW46588 Da

ACADM Antibody (C-term) - Additional info

Gene ID 34
Other Names
ACADM; Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Target/Specificity
This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the C-terminal region of human ACADM.
Dilution
WB~~1:100~500
FC~~1:10~50
IF~~1:10~50
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ACADM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

ACADM Antibody (C-term) - Protein Information

Name ACADM
Function
This enzyme is specific for acyl chain lengths of 4 to 16
Cellular Location
Mitochondrion matrix.

ACADM Antibody (C-term) - Related products

AP6827b: ACADM Antibody (C-term)

AP6827c: ACADM Antibody (Center)

RI10041: ACADM predesign siRNA

DC01760: Human ACADM cDNA Clone

LY11479a: ACADM Over-expression Lysate

BP6827b: ACADM Antibody (C-term) Blocking Peptide

BP6827c: ACADM Antibody (Center) Blocking Peptide

AJ1007a: ACADM Antibody

AJ1300a: FKBP12 Antibody

AF1016a: ACADM Antibody

ACADM Antibody (C-term) - Application data

  • Western blot analysis of ACADM Antibody (C-term) (Cat. #AP6827b) in Jurkat cell line lysates (35ug/lane). ACADM (arrow) was detected using the purified Pab.

  • ACADM Antibody (C-term) (Cat. #AP6827b) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Confocal immunofluorescent analysis of ACADM Antibody (C-term)(Cat#AP6827b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Formalin-fixed and paraffin-embedded human brain tissue reacted with ACADM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ACADM Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

ACADM is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.

REFERENCES

Ferreira,A.C., et.al., Genet. Mol. Res. 8 (2), 487-493 (2009)