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>   home   >   Products   >   Primary Antibodies   >   BCKDHA Antibody (C-term)   

BCKDHA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BCKDHA Antibody (C-term) AP6830b
    Western blot analysis of BCKDHA Antibody (C-term) (Cat. #AP6830b) in Jurkat cell line lysates (35ug/lane). BCKDHA (arrow) was detected using the purified Pab.
  • IHC-P - BCKDHA Antibody (C-term) AP6830b
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCKDHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - BCKDHA Antibody (C-term) AP6830b
    BCKDHA Antibody (C-term) (Cat. #AP6830b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12694
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50471 Da
Antigen Region 362-390 aa
Additional Information
Gene ID 593
Other Names 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA
Target/Specificity This BCKDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the C-terminal region of human BCKDHA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCKDHA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Cellular Location Mitochondrion matrix.
Citations (0)

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The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component.


Flaschker,N.,, J. Inherit. Metab. Dis. 30 (6), 903-909 (2007)

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Cat# AP6830b
(40 western blots)
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