|Application ||WB, IHC-P, E|
|Other Accession||O54791, A7YY73|
|Calculated MW||17760 Da|
|Antigen Region||66-92 aa|
|Other Names||Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF|
|Target/Specificity||This MAFF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the Central region of human MAFF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAFF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:8932385). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed:8932385, PubMed:16549056). May be a transcriptional enhancer in the up- regulation of the oxytocin receptor gene at parturition (PubMed:10527846).|
|Tissue Location||Expressed in the term myometrium and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. This protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription.
Kataoka,K., et.al., J. Biol. Chem. 276 (1), 819-826 (2001)
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