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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   FHL1 Antibody (C-term)   

FHL1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - FHL1 Antibody (C-term) AP6852b
    Western blot analysis of FHL1 Antibody (C-term) (Cat. #AP6852b) in NIH-3T3 cell line lysates (35ug/lane). FHL1 (arrow) was detected using the purified Pab.
  • IHC-P - FHL1 Antibody (C-term) AP6852b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with FHL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - FHL1 Antibody (C-term) AP6852b
    Flow cytometric analysis of widr cells using FHL1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - FHL1 Antibody (C-term) AP6852b
    Western blot analysis of FHL1 (arrow) using rabbit polyclonal FHL1 Antibody (C-term) (Cat. #AP6852b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FHL1 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13642
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36263 Da
Antigen Region 214-240 aa
Additional Information
Gene ID 2273
Other Names Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1, FHL1, SLIM1
Target/Specificity This FHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-240 amino acids from the C-terminal region of human FHL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFHL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FHL1
Synonyms SLIM1
Function May have an involvement in muscle development or hypertrophy.
Cellular Location Isoform 1: Cytoplasm. Isoform 2: Nucleus. Cytoplasm, cytosol. Note=Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes
Tissue Location Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.
Research Areas
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FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.


Gueneau,L.,, Am. J. Hum. Genet. 85 (3), 338-353 (2009)

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$ 295.00
$ 99.00
Cat# AP6852b
(40 western blots)
Availability: In Stock
Bulk Size
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