|Application ||WB, IHC-P, E|
|Other Accession||Q3YLA6, Q6PDM2, Q5ZML3, Q0VCY7|
|Predicted||Bovine, Chicken, Mouse, Pig|
|Calculated MW||27745 Da|
|Antigen Region||11-38 aa|
|Other Names||Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1|
|Target/Specificity||This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-38 amino acids from the N-terminal region of human SFRS1.|
|Precautions||SFRS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ASF, SF2, SF2P33, SFRS1|
|Function||Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.|
|Cellular Location||Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins.
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007)
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