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>   home   >   Products   >   Primary Antibodies   >   SFRS1 Antibody (C-term)   

SFRS1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SFRS1 Antibody (C-term) AP6857b
    Western blot analysis of SFRS1 Antibody (C-term) (Cat. #AP6857b) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.
  • IHC-P - SFRS1 Antibody (C-term) AP6857b
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - SFRS1 Antibody (C-term) AP6857b
    Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term) (Cat. #AP6857b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.
  • FC - SFRS1 Antibody (C-term) AP6857b
    SFRS1 Antibody (C-term) (Cat. #AP6857b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q07955
Other Accession Q3YLA6, Q6PDM2, Q5ZML3, Q0VCY7, Q6NYA0, Q7SXP4
Reactivity Human
Predicted Zebrafish, Bovine, Chicken, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 27745 Da
Antigen Region 158-186 aa
Additional Information
Gene ID 6426
Other Names Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1
Target/Specificity This SFRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human SFRS1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSFRS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SRSF1
Synonyms ASF, SF2, SF2P33, SFRS1
Function Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
Cellular Location Cytoplasm. Nucleus speckle. Note=In nuclear speckles. Shuttles between the nucleus and the cytoplasm
Citations (0)

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SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins.


Sugiyama,N.,, Mol. Cell Proteomics 6 (6), 1103-1109 (2007)

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$ 295.00
$ 99.00
Cat# AP6857b
(40 western blots)
Availability: In Stock
Bulk Size
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