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ASPA Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6859a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP6859a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ASPA Antibody (N-term) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | P45381 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35735 Da |
ASPA Antibody (N-term) - Additional info | |
| Gene ID 443 | |
| Other Names ASPA; ACY2; ASP; Aspartoacylase; Aminoacylase-2 | |
| Target/Specificity This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~118 amino acids from the N-terminal region of human ASPA. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ASPA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ASPA Antibody (N-term) - Protein Information | |
| Name ASPA | |
| Synonyms ACY2, ASP | |
| Function Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids | |
| Cellular Location Cytoplasm. Nucleus (By similarity). | |
| Tissue Location Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver | |
ASPA Antibody (N-term) - Related products
AP6859a: ASPA Antibody (N-term)
DC05904: Human ASPA cDNA Clone
LY10803a: ASPA Over-expression Lysate
ASPA Antibody (N-term) - Application data
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Western blot analysis of ASPA Antibody (N-term) (Cat. #AP6859a) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ASPA (arrow) using rabbit polyclonal ASPA Antibody (N-term) (Cat. #AP6859a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASPA gene.
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Confocal immunofluorescent analysis of ASPA Antibody (N-term)(Cat. #AP6859a) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ASPA Antibody (N-term) - Research Areas
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BACKGROUND
ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.
REFERENCES
Bitto,E., et.al., Proc. Natl. Acad. Sci. U.S.A. 104 (2), 456-461 (2007)
