- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
AIK Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Alternative | |||
| AP6862a | AP7002b, AP7002a, AP7900a | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
AIK Antibody (N-term) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | O14965 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 45809 Da |
AIK Antibody (N-term) - Additional info | |
| Gene ID 6790 | |
| Other Names AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A | |
| Target/Specificity This AIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110~139 amino acids from the N-terminal region of human AIK. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AIK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AIK Antibody (N-term) - Protein Information | |
| Name AURKA | |
| Function Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53 Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis | |
| Cellular Location Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase. Moves to the midbody during both telophase and cytokinesis. Associates with both the pericentriolar material (PCM) and centrioles | |
| Tissue Location Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines | |
AIK Antibody (N-term) - Related products
AP6862a: AIK Antibody (N-term)
AP6863b: AURKA Antibody (C-term)
AP7002a: Aurora-A Antibody (N-term E107)
AP7002b: Aurora-A Antibody (N-term S89)
AP7002c: Aurora-A Antibody (C-term)
AP7900a: AIK Antibody (N-term)
RI10498: AURKA predesign siRNA
DC11922: Human AURKA cDNA Clone
LY10088a: AURKA Over-expression Lysate
BP6862a: AIK Antibody (N-term) Blocking Peptide
BP6863b: AURKA Antibody (C-term) Blocking Peptide
BP7002a: Aurora-A Antibody (N-term E107) Blocking Peptide
BP7002b: Aurora-A Antibody (N-term S89) Blocking Peptide
BP7002c: Aurora-A Antibody (C-term) Blocking Peptide
BP7900a: AIK Antibody (N-term) Blocking Peptide
AT4091a: STK6 Antibody (monoclonal) (M01)
AIK Antibody (N-term) - Application data
-
Western blot analysis of AIK Antibody (N-term) (Cat. #AP6862a) in Hela cell line lysates (35ug/lane). AIK (arrow) was detected using the purified Pab.
-
Western blot analysis of AIK (arrow) using rabbit polyclonal AIK Antibody (N-term) (Cat. #AP6862a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2) (Origene Technologies).
-
Formalin-fixed and paraffin-embedded human testis reacted with AIK Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Flow cytometric analysis of jurkat cells using AIK Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Immunofluorescence analysis of AIK Antibody (N-term) with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
AIK Antibody (N-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
AIK is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. This protein is found at the centrosome in interphase cells and at the spindle poles in mitosis.
REFERENCES
Matsuoka,S., et.al., Science 316 (5828), 1160-1166 (2007)
