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GAPVD1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6872b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
GAPVD1 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | Q14C86 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 164980 Da |
GAPVD1 Antibody (C-term) - Additional info | |
| Gene ID 26130 | |
| Other Names GAPVD1; GAPEX5; KIAA1521; RAP6; GTPase-activating protein and VPS9 domain-containing protein 1; GAPex-5; Rab5-activating protein 6 | |
| Target/Specificity This GAPVD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1045~1075 amino acids from the C-terminal region of human GAPVD1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions GAPVD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
GAPVD1 Antibody (C-term) - Protein Information | |
| Name GAPVD1 | |
| Synonyms GAPEX5, KIAA1521, RAP6 | |
| Function Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras | |
| Cellular Location Membrane; Peripheral membrane protein. Endosome. Note=Recruited to the plasma membrane by TRIP10/CIP4 in response to insulin | |
GAPVD1 Antibody (C-term) - Related products
AP6872b: GAPVD1 Antibody (C-term)
GAPVD1 Antibody (C-term) - Application data
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Western blot analysis of GAPVD1 Antibody (C-term) (Cat. #AP6872b) in 293 cell line lysates (35ug/lane). GAPVD1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with GAPVD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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GAPVD1 Antibody (C-term) (Cat. #AP6872b) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAPVD1 Antibody (C-term) - Research Areas
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BACKGROUND
GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.
REFERENCES
Matsuoka,S., et.al., Science 316 (5828), 1160-1166 (2007)
