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GLUL Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - GLUL Antibody (N-term) AP6892a
    AP6892a staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    AP6892a staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    AP6892a staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    AP6892a staining GLUL in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: MOLT-4 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    Anti-GLUL Antibody (N-term)at 1:1000 dilution + Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: mouse cerebellum lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole cell lysates Lane 2: mouse cerebellum lysates Lane 3: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLUL Antibody (N-term) AP6892a
    All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse cerebellum lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    Immunohistochemical analysis of paraffin-embedded H. liver section using GLUL Antibody (N-term)(Cat#AP6892a). AP6892a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    Immunohistochemical analysis of paraffin-embedded H. liver section using GLUL Antibody (N-term)(Cat#AP6892a). AP6892a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from HT-29 cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate(from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate(from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from Hela, Raji, Jurkat, HT-29 cell line (from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from Jurkat, Raji, HT-29 cell line (from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from Hela, Jurkat, HT-29 cell line (from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of lysates from Hela, Jurkat, HT-29, MOLT-4 cell line (from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GLUL Antibody (N-term) AP6892a
    Western blot analysis of GLUL Antibody (N-term) (Cat. #AP6892a) in mouse cerebellum tissue lysates (35ug/lane). GLUL (arrow) was detected using the purified Pab.
  • IHC-P - GLUL Antibody (N-term) AP6892a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with GLUL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P15104
Other Accession P46410, P15105, Q4R7U3, P16580, P15103
Reactivity Human, Mouse
Predicted Bovine, Chicken, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42064 Da
Antigen Region 70-96 aa
Additional Information
Gene ID 2752
Other Names Glutamine synthetase, GS, Glutamate decarboxylase, Glutamate--ammonia ligase, GLUL, GLNS
Target/Specificity This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL.
Dilution IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLUL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLUL
Synonyms GLNS
Function This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Cellular Location Cytoplasm. Mitochondrion.
Research Areas
Citations (0)

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Background

GLUL belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling.

References

Di Tommaso,L., et.al., J. Hepatol. 50 (4), 746-754 (2009)

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$ 295.00
$ 99.00
Cat# AP6892a
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