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GSTM1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GSTM1 Antibody (C-term) AP6896b
    Western blot analysis of lysates from Jurkat, U251 cell line and human liver tissue (from left to right), using GSTM1 Antibody (C-term) (Cat. #AP6896b). AP6896b was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • IHC-P - GSTM1 Antibody (C-term) AP6896b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTM1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GSTM1 Antibody (C-term) AP6896b
    GSTM1 Antibody (C-term) (Cat. #AP6896b) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P09488
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25712 Da
Antigen Region 184-211 aa
Additional Information
Gene ID 2944
Other Names Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GSTM1, GST1
Target/Specificity This GSTM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-211 amino acids from the C-terminal region of human GSTM1.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTM1 (HGNC:4632)
Synonyms GST1
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).
Cellular Location Cytoplasm.
Tissue Location Liver (at protein level).
Research Areas
Citations (0)
citation

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Background

GSTM1 is a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs.

References

Kostrykina,N.A., et.al., Bull. Exp. Biol. Med. 148 (1), 89-93 (2009)

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$ 365.00
$ 140.00
Cat# AP6896b
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