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> home > Products > Antibodies > Biological Process > Metabolic Process > ASS Antibody (C-term)

ASS Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Catalog #	Size	Availability	Price
AP6899b	0.1 mg 400 ul	In Stock	$ 255.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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ASS Antibody (C-term) - Product info
Application	WB, IHC, FC, IF Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P00966
Reactivity	Human
Concentration	0.25 mg/ml
Isotype	Rabbit Ig
Calculated MW	46530 Da
ASS Antibody (C-term) - Additional info
Gene ID 445
Other Names ASS1; ASS; Argininosuccinate synthase; Citrulline--aspartate ligase
Target/Specificity This ASS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383~412 amino acids from the C-terminal region of human ASS.
Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions ASS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
ASS Antibody (C-term) - Protein Information
Name ASS1
Synonyms ASS
Function Is indirectly involved in the control of blood pressure (By similarity)

ASS Antibody (C-term) - Related products

AP12606b: ASS1 Antibody (C-term)

AP6829c: ASS Antibody (Center)

AP6899b: ASS Antibody (C-term)

RI10443: ASS1 predesign siRNA

DC05120: Human ASS1 cDNA Clone

LY10938a: ASS1 Over-expression Lysate

BP6829c: ASS Antibody (Center) Blocking Peptide

BP6899b: ASS Antibody (C-term) Blocking Peptide

AF1097a: Argininosuccinate synthetase 1 Antibody

ASS Antibody (C-term) - Application data

Western blot analysis of ASS Antibody (C-term) (Cat. #AP6899b) in HepG2 cell line lysates (35ug/lane). ASS (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ASS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ASS Antibody (C-term) (Cat. #AP6899b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ASS Antibody (C-term)(Cat#AP6899b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

ASS Antibody (C-term) - Research Areas

Antibodies Biological Process Metabolic Process Primary Metabolic Process Cellular Metabolic Process Cellular Process Cellular Ketone Metabolic Process Oxoacid Metabolic Process Carboxylic Acid Metabolic Process Organic Acid Metabolic Process Cellular Amino Acid And Derivative Metabolic Process Cellular Amine Metabolic Process Amine Metabolic Process Cellular Amino Acid Metabolic Process Organic Acid Biosynthetic Process Carboxylic Acid Biosynthetic Process Nitrogen Compound Biosynthetic Process Biosynthetic Process Cellular Biosynthetic Process Cellular Amide Metabolic Process Nitrogen Compound Metabolic Process Glutamine Family Amino Acid Metabolic Process Amine Biosynthetic Process Cellular Nitrogen Compound Metabolic Process Cellular Compartment Cytoplasm Intracellular Part Intracellular Molecular Function Catalytic Activity Protein Binding Purine Nucleotide Binding Nucleotide Binding Ribonucleotide Binding Purine Ribonucleotide Binding Purine Nucleoside Binding Nucleoside Binding Adenyl Nucleotide Binding

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

ASS catalyzes the penultimate step of the arginine biosynthetic pathway.

REFERENCES

Kohler,E.S., et.al., BMC Dev. Biol. 8, 107 (2008)