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RAGE Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAGE Antibody (Center) AP6910c
    Western blot analysis of lysates from HepG2 cell line and human lung tissue lysates (from left to right), using RAGE Antibody (Center)(Cat. #AP6910c). AP6910c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - RAGE Antibody (Center) AP6910c
    Western blot analysis of lysate from human lung tissue lysate, using RAGE Antibody (Center)(Cat. #AP6910c). AP6910c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • IF - RAGE Antibody (Center) AP6910c
    Confocal immunofluorescent analysis of RAGE Antibody (Center)(Cat#AP6910c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9UQ07
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48014 Da
Antigen Region 206-234 aa
Additional Information
Gene ID 5891
Other Names MAPK/MAK/MRK overlapping kinase, MOK protein kinase, Renal tumor antigen 1, RAGE-1, MOK, RAGE, RAGE1
Target/Specificity This RAGE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-234 amino acids from the Central region of human RAGE.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAGE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MOK
Synonyms RAGE, RAGE1
Function Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9WVS4}. Cell projection, cilium {ECO:0000250|UniProtKB:Q9WVS4}. Nucleus {ECO:0000250|UniProtKB:Q9WVS4}
Tissue Location Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina
Research Areas
Citations (0)

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Background

RAGE is able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

References

Peng,W.H., et.al., Arch. Med. Res. 40 (5), 393-398 (2009)

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$ 295.00
$ 99.00
Cat# AP6910c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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