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SAR1A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SAR1A Antibody (Center) AP6915c
    Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody (Center)(Cat. #AP6915c).AP6915c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • IHC-P - SAR1A Antibody (Center) AP6915c
    Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - SAR1A Antibody (Center) AP6915c
    Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9NR31
Other Accession Q52NJ3, P36536, Q3T0D7
Reactivity Human
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22367 Da
Antigen Region 122-149 aa
Additional Information
Gene ID 56681
Other Names GTP-binding protein SAR1a, COPII-associated small GTPase, SAR1A, SAR1, SARA, SARA1
Target/Specificity This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human SAR1A.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAR1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAR1A
Synonyms SAR1, SARA, SARA1
Function Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Cellular Location Endoplasmic reticulum. Golgi apparatus
Research Areas
Citations (0)

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Background

SAR1A is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity) and required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).

References

Morgan,A.R., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (6), 762-770(2007)

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$ 295.00
$ 99.00
Cat# AP6915c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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