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TGM4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TGM4 Antibody (Center) AP6918c
    All lanes : Anti-TGM4 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: PC-3 whole cell lysateLane 2: Hela whole cell lysate Lane 3: LNCap whole cell lysate Lane 4: CCRF-CEM whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: Mouse prostate whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TGM4 Antibody (Center) AP6918c
    Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TGM4 Antibody (Center) AP6918c
    Flow cytometric analysis of CEM cells using TGM4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P49221
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 77145 Da
Antigen Region 135-164 aa
Additional Information
Gene ID 7047
Other Names Protein-glutamine gamma-glutamyltransferase 4, Fibrinoligase, Prostate transglutaminase, Prostate-specific transglutaminase, Transglutaminase P, TG(P), TGP, TGase P, Transglutaminase-4, TGase-4, TGM4
Target/Specificity This TGM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human TGM4.
Dilution WB~~1:1000-1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGM4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGM4
Function Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
Tissue Location Prostate.
Research Areas
Citations (0)
citation

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Background

TGM4is associated with the mammalian reproductive process and catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.

References

Jiang,W.G., et.al., Microvasc. Res. 77 (2), 150-157 (2009)

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$ 365.00
$ 140.00
Cat# AP6918c
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