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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MyoGEF Antibody (N-term)   

MyoGEF Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MyoGEF Antibody (N-term) AP6922a
    Western blot analysis of MyoGEF Antibody (N-term) (Cat. #AP6922a) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.
  • IHC-P - MyoGEF Antibody (N-term) AP6922a
    Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - MyoGEF Antibody (N-term) AP6922a
    Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q3KR16
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 88960 Da
Antigen Region 60-87 aa
Additional Information
Gene ID 55200
Other Names Pleckstrin homology domain-containing family G member 6, PH domain-containing family G member 6, Myosin-interacting guanine nucleotide exchange factor, MyoGEF, PLEKHG6
Target/Specificity This MyoGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-87 amino acids from the N-terminal region of human MyoGEF.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyoGEF Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEKHG6
Function Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.
Cellular Location Cell projection, microvillus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow. Note=During mitosis, localizes to the spindle pole, central spindle and cleavage furrow (PubMed:16721066). In epithelial cells, recruited to the apical membrane by EZR where it participates in macropinocytosis (PubMed:17881735).
Tissue Location Highest expression in the placenta. Low levels in small intestine, lung, liver, kidney, thymus and heart
Research Areas
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Background

Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG. Its part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, It is required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, it is required for normal macropinocytosis.

References

D'Angelo,R., et.al., Mol. Biol. Cell 18 (12), 4780-4793 (2007)

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Cat# AP6922a
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(40 western blots)
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