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OTC Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - OTC Antibody (Center) AP6928c
    Anti-OTC Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - OTC Antibody (Center) AP6928c
    OTC Antibody (Center) (Cat.# AP6928c) western blot analysis in SW620 cell line and mouse liver,rat liver lysates (35ug/lane).This demonstrates the OTC antibody detected the OTC protein (arrow).
  • IHC-P - OTC Antibody (Center) AP6928c
    Formalin-fixed and paraffin-embedded human hepatoarcinoma with OTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - OTC Antibody (Center) AP6928c
    Flow cytometric analysis of ATDC5 cells using OTC Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00480
Other Accession P00481, O19072, P11725, Q9N1U7, P84010
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39935 Da
Antigen Region 71-98 aa
Additional Information
Gene ID 5009
Other Names Ornithine carbamoyltransferase, mitochondrial, Ornithine transcarbamylase, OTCase, OTC
Target/Specificity This OTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human OTC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTC
Cellular Location Mitochondrion matrix.
Tissue Location Mainly expressed in liver and intestinal mucosa
Research Areas
Citations (0)

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OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia.


Hansmannel,F.,, Neurosci. Lett. 449 (1), 76-80 (2009)

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$ 295.00
$ 99.00
Cat# AP6928c
(40 western blots)
Availability: In Stock
Bulk Size
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