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NDUFS4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P-Leica, FC, WB, E |
|---|---|
| Primary Accession | O43181 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20108 Da |
| Antigen Region | 131-160 aa |
| Gene ID | 4724 |
|---|---|
| Other Names | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, Complex I-18 kDa, CI-18 kDa, Complex I-AQDQ, CI-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, NDUFS4 |
| Target/Specificity | This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human NDUFS4. |
| Dilution | WB~~1:2000 IHC-P-Leica~~1:250 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NDUFS4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NDUFS4 |
|---|---|
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Cellular Location | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note=The interaction with BCAP31 mediates mitochondria localization. |
Provided below are standard protocols that you may find useful for product applications.
Background
NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
References
Panelli,D., et.al., Biochimie 90 (10), 1452-1460 (2008)
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