|Application ||WB, FC, E|
|Other Accession||Q8CFN2, Q007T2, P60766, Q4R4R6, Q90694, Q2KJ93|
|Reactivity||Human, Mouse, Rat|
|Predicted||Bovine, Chicken, Monkey, Mouse, Pig, Rat|
|Calculated MW||21259 Da|
|Antigen Region||106-134 aa|
|Other Names||Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42|
|Target/Specificity||This CDC42 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the Central region of human CDC42.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDC42 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle. Midbody. Note=Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome|
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Provided below are standard protocols that you may find useful for product applications.
CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex.
Landry,M.C., et.al., Mol. Biol. Cell 20 (18), 4091-4106 (2009)
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