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IGFBP4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IGFBP4 Antibody (N-term) AP6941a
    Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution + Rat ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IGFBP4 Antibody (N-term) AP6941a
    All lanes : Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Mouse ovary lysate Lane 3: Rat ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - IGFBP4 Antibody (N-term) AP6941a
    IGFBP4 Antibody (N-term) (Cat. #AP6941a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P22692
Other Accession P24854, Q05716
Reactivity Human, Mouse, Rat
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 27934 Da
Antigen Region 66-92 aa
Additional Information
Gene ID 3487
Other Names Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4
Target/Specificity This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the N-terminal region of human IGFBP4.
Dilution WB~~1:2000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGFBP4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGFBP4
Synonyms IBP4
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

IGFBP4 has an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

References

Durai,R., et.al., Colorectal Dis 9 (7), 625-631 (2007)
Laursen,L.S., et.al., Mol. Endocrinol. 21 (5), 1246-1257 (2007)

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$ 295.00
$ 99.00
Cat# AP6941a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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