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His Tag AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6947a | 0.1 mg 400 ul | In Stock | $ 195.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP6947a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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His Tag Antibody - Product info | |
| Application | WB
|
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 36526 Da |
His Tag Antibody - Additional info | |
| Target/Specificity Poly-HIS peptide were used to produced this antibody. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions His Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
His Tag Antibody - Application data
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Western blot analysis of His Tag Antibody (Cat. #AP6947a) in his-tag TBB1 protein (35ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.
His Tag Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
REFERENCES
Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.
