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PIK4CB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PIK4CB Antibody (Center) AP6948c
    Western blot analysis of PIK4CB Antibody (Center) (Cat. #AP6948c) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.
  • IHC-P - PIK4CB Antibody (Center) AP6948c
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PIK4CB Antibody (Center) AP6948c
    PIK4CB Antibody (Center) (Cat. #AP6948c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UBF8
Other Accession O08561, Q8BKC8, Q49GP3, O02810
Reactivity Human
Predicted Bovine, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91379 Da
Antigen Region 519-548 aa
Additional Information
Gene ID 5298
Other Names Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KB, PIK4CB
Target/Specificity This PIK4CB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the Central region of human PIK4CB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIK4CB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI4KB
Synonyms PIK4CB
Function Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
Cellular Location Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus. Cytoplasm, perinuclear region. Note=Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex
Tissue Location Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
Research Areas
Citations (0)

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PIK4CB phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.


Jeganathan,S.,, Mol. Cell. Biol. 28 (14), 4549-4561 (2008) Pizarro-Cerda,J.,, Cell. Microbiol. 9 (10), 2381-2390 (2007)

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Cat# AP6948c
(40 western blots)
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