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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CBS Antibody (Center)   

CBS Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - CBS Antibody (Center) AP6959c
    Overlay histogram showing Hela cells stained with AP6959c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6959c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - CBS Antibody (Center) AP6959c
    Anti-CBS Antibody (Center) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CBS Antibody (Center) AP6959c
    Immunohistochemical analysis of paraffin-embedded H. brain section using CBS Antibody (Center)(Cat#AP6959c). AP6959c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - CBS Antibody (Center) AP6959c
    CBS Antibody (Center) (Cat. #AP6959c) western blot analysis in Raji cell line, rat brain and cerebellum tissue lysates (35ug/lane).This demonstrates the CBS antibody detected the CBS protein (arrow).
  • IHC-P - CBS Antibody (Center) AP6959c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CBS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - CBS Antibody (Center) AP6959c
    Confocal immunofluorescent analysis of CBS Antibody (Center)(Cat#AP6959c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35520
Other Accession Q58H57
Reactivity Human, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60587 Da
Antigen Region 301-330 aa
Additional Information
Gene ID 875
Other Names Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Target/Specificity This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the Central region of human CBS.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBS
Function Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney
Research Areas
Citations (0)

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CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.


Ravel,C.,, PLoS ONE 4 (8), E6540 (2009)

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$ 295.00
$ 99.00
Cat# AP6959c
(40 western blots)
Availability: In Stock
Bulk Size
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