- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
NOS3 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP6963c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
NOS3 Antibody (Center) - Product info | |
| Application | WB, IHC, FC, IF
|
| Primary Accession | P29474 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 133289 Da |
NOS3 Antibody (Center) - Additional info | |
| Gene ID 4846 | |
| Other Names NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III | |
| Target/Specificity This NOS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 713~743 amino acids from the Center region of human NOS3. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NOS3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NOS3 Antibody (Center) - Protein Information | |
| Name NOS3 | |
| Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets | |
| Cellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity | |
| Tissue Location Platelets, placenta, liver and kidney. | |
NOS3 Antibody (Center) - Related products
AP11828a: eNos Antibody (S1177)
AP6963a: NOS3 Antibody (N-term)
AP6963c: NOS3 Antibody (Center)
BP3665a: Phospho-eNos-S1177 Blocking Peptide
BP6963a: NOS3 Antibody (N-term) Blocking Peptide
NOS3 Antibody (Center) - Application data
-
Western blot analysis of NOS3 Antibody (Center) (Cat. #AP6963c) in HepG2 cell line lysates (35ug/lane). NOS3 (arrow) was detected using the purified Pab.
-
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Flow cytometric analysis of HepG2 cells using NOS3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Confocal immunofluorescent analysis of NOS3 (Center)(Cat#AP6963c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
-
Confocal immunofluorescent analysis of NOS3 Antibody (Center)(Cat#AP6963c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
NOS3 Antibody (Center) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
REFERENCES
Rikova,K., et.al., Cell 131 (6), 1190-1203 (2007)
