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Rab5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| FC, IF, IHC-P, WB, E |
|---|---|
| Primary Accession | P20339 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 23659 Da |
| Antigen Region | 182-211 aa |
| Gene ID | 5868 |
|---|---|
| Other Names | Ras-related protein Rab-5A, RAB5A, RAB5 |
| Target/Specificity | This Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human Rab5. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Rab5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RAB5A |
|---|---|
| Synonyms | RAB5 |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid- anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle {ECO:0000250|UniProtKB:P18066}. Membrane Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q9CQD1}. Endosome membrane Note=Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable) {ECO:0000269|PubMed:17081065, ECO:0000305} |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Rab5 is required for the fusion of plasma membranes and early endosomes.
References
Farnsworth,C.C., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (25), 11963-11967 (1994)
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$ 365.00
$ 140.00
Cat# AP6974B
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