|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||23659 Da|
|Antigen Region||182-211 aa|
|Other Names||Ras-related protein Rab-5A, RAB5A, RAB5|
|Target/Specificity||This Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human Rab5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rab5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Note=Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms|
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Provided below are standard protocols that you may find useful for product applications.
Rab5 is required for the fusion of plasma membranes and early endosomes.
Farnsworth,C.C., et.al., Proc. Natl. Acad. Sci. U.S.A. 91 (25), 11963-11967 (1994)
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