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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   LIF Antibody (Center)   

LIF Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LIF Antibody (Center) AP6981C
    Anti-LIF Antibody (Center) at 1:2000 dilution + KG-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LIF Antibody (Center) AP6981C
    Western blot analysis of lysates from rat PC-12 cell line, mouse spleen tissue lysate (from left to right), using LIF Antibody (Center)(Cat. #AP6981c). AP6981c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - LIF Antibody (Center) AP6981C
    Western blot analysis of LIF Antibody (Center) (Cat. #AP6981c) in CEM cell line lysates (35ug/lane). LIF (arrow) was detected using the purified Pab.
  • WB - LIF Antibody (Center) AP6981C
    Western blot analysis of LIF (arrow) using rabbit polyclonal LIF Antibody (Center) (Cat. #AP6981c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LIF gene.
  • IHC-P - LIF Antibody (Center) AP6981C
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - LIF Antibody (Center) AP6981C
    Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15018
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22008 Da
Antigen Region 72-101 aa
Additional Information
Gene ID 3976
Other Names Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, LIF, HILDA
Target/Specificity This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human LIF.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIF
Synonyms HILDA
Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Cellular Location Secreted.
Research Areas
Citations (0)

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LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.


Novotny,Z.,, Folia Biol. (Praha) 55 (3), 92-97 (2009)

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Cat# AP6981C
(40 western blots)
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