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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD28 Antibody (C-term)   

CD28 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD28 Antibody (C-term) AP6982B
    Western blot analysis of CD28 Antibody (C-term) (Cat. #AP6982b) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.
  • IF - CD28 Antibody (C-term) AP6982B
    Confocal immunofluorescent analysis of CD28 Antibody (C-term) (Cat. #AP6982b) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P10747
Other Accession P42069
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 25066 Da
Antigen Region 182-208 aa
Additional Information
Gene ID 940
Other Names T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28
Target/Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD28 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD28
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in T-cells and plasma cells, but not in less mature B-cells
Research Areas
Citations (0)

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Background

CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.

References

Zocchi,M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992)

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Cat# AP6982B
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(40 western blots)
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