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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GRK5 Antibody (C-term)   

GRK5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GRK5 Antibody (C-term) AP7007a
    All lanes : Anti-GRK5 Antibody (N574) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GRK5 Antibody (C-term) AP7007a
    All lanes : Anti-GRK5 Antibody (N574) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Ramos whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GRK5 Antibody (C-term) AP7007a
    Western blot analysis of anti-GRK5 Pab (Cat. #AP7007a) in mouse brain tissue lysate. GRK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - GRK5 Antibody (C-term) AP7007a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - GRK5 Antibody (C-term) AP7007a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK5 Antibody (C-term) (Cat.#AP7007a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P34947
Other Accession P43249
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67787 Da
Antigen Region 559-590 aa
Additional Information
Gene ID 2869
Other Names G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5, GRK5, GPRK5
Target/Specificity This GRK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-590 amino acids from the C-terminal region of human GRK5.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRK5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRK5
Synonyms GPRK5
Function Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Cellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion
Tissue Location Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney
Research Areas
Citations (0)

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Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

References

Pronin, A.N., et al., J. Biol. Chem. 275(34):26515-26522 (2000).
Pronin, A.N., et al., J. Biol. Chem. 273(47):31510-31518 (1998).
Nagayama, Y., et al., J. Biol. Chem. 271(17):10143-10148 (1996).
Kunapuli, P., et al., J. Biol. Chem. 269(2):1099-1105 (1994).
Kunapuli, P., et al., Proc. Natl. Acad. Sci. U.S.A. 90(12):5588-5592 (1993).

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$ 295.00
$ 99.00
Cat# AP7007a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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