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PKC nu Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7025b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP7025b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
PKC nu Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O94806 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 100471 Da |
PKC nu Antibody (C-term) - Additional info | |
| Gene ID 23683 | |
| Other Names PRKD3; EPK2; PRKCN; Serine/threonine-protein kinase D3; Protein kinase C nu type; Protein kinase EPK2; nPKC-nu | |
| Target/Specificity This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PKC nu Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PKC nu Antibody (C-term) - Protein Information | |
| Name PRKD3 | |
| Synonyms EPK2, PRKCN | |
| Function Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity) | |
| Cellular Location Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation | |
| Tissue Location Ubiquitous. | |
PKC nu Antibody (C-term) - Related products
AP7025b: PKC nu Antibody (C-term)
RI14232: PRKD3 predesign siRNA
DC06149: Human PRKD3 cDNA Clone
PKC nu Antibody (C-term) - Application data
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Western blot analysis of anti-PKCnu C-term Pab (Cat. #AP7025b) in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (AP7025a) and C-term (AP7025b) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using AP7025a and AP7025b antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PKC nu Antibody (C-term) - Research Areas
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BACKGROUND
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
REFERENCES
Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004). Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003). Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003). Bennasser, Y., et al., Virology 303(1):174-180 (2002). Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).
