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AKT3 Antibody (T447)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7030e | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
AKT3 Antibody (T447) - Product info | |
| Application | WB
|
| Primary Accession | Q9Y243 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 55775 Da |
AKT3 Antibody (T447) - Additional info | |
| Gene ID 10000 | |
| Other Names AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; RAC-PK-gamma; STK-2 | |
| Target/Specificity This AKT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T447 of human AKT3. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AKT3 Antibody (T447) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AKT3 Antibody (T447) - Protein Information | |
| Name AKT3 | |
| Synonyms PKBG | |
| Function AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis | |
| Cellular Location Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation | |
| Tissue Location In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney | |
AKT3 Antibody (T447) - Related products
AM1849a: AKT3 Antibody (Ascites)
AP3468a: Phospho-AKT3-S472 Antibody
AP7030a: AKT3 Antibody (N-term)
AP7030c: AKT3 Antibody (Center)
AP8647b: AIK3 Antibody (C-term)
BP3468a: Phospho-AKT3-S472 Antibody Blocking Peptide
BP7030c: AKT3 Antibody (Center) Blocking Peptide
BP7030d: AKT3 Antibody (S472) Blocking Peptide
BP7030e: AKT3 Antibody (T447) Blocking Peptide
BP8647b: AIK3 Antibody (C-term) Blocking Peptide
AKT3 Antibody (T447) - Application data
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Western blot analysis of AKT3 (arrow) using rabbit polyclonal AKT3 Antibody (T447). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies).
AKT3 Antibody (T447) - Research Areas
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BACKGROUND
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
REFERENCES
Xu, Z., et al., Biochem. Biophys. Res. Commun. 312(2):388-396 (2003). Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).
