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PDK3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PDK3 Antibody (N-term) AP7040a
    Western blot analysis of PDK3 (arrow) using rabbit polyclonal PDK3 Antibody (N-term) (Cat# AP7040a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK3 gene (Lane 2) (Origene Technologies).
    detail
  • WB - PDK3 Antibody (N-term) AP7040a
    PDK3 Antibody (M1) (Cat. #AP7040a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDK3 antibody detected the PDK3 protein (arrow).
    detail
  • SPECIFICATION
  • CITATIONS: 2
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15120
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46939 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 5165
Other Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, Pyruvate dehydrogenase kinase isoform 3, PDK3, PDHK3
Target/Specificity This PDK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDK3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDK3
Synonyms PDHK3
Function Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
Cellular Location Mitochondrion matrix.
Tissue Location Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain.
Research Areas
Citations ( 0 )

Background

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

References

Baker, J.C., et al., J. Biol. Chem. 275(21):15773-15781 (2000). Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995).

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$ 182.50
$ 70.00
Cat# AP7040a
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