- CITATIONS: 2
|Application ||WB, E|
|Calculated MW||46939 Da|
|Antigen Region||1-30 aa|
|Other Names||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, Pyruvate dehydrogenase kinase isoform 3, PDK3, PDHK3|
|Target/Specificity||This PDK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDK3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.|
|Cellular Location||Mitochondrion matrix.|
|Tissue Location||Expressed in heart, skeletal muscle, spinal cord, as well as fetal and adult brain|
Provided below are standard protocols that you may find useful for product applications.
PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Baker, J.C., et al., J. Biol. Chem. 275(21):15773-15781 (2000). Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995).
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